Journal: bioRxiv

Article Title: Kidney cystogenesis in embryonic- and adult-onset ADPKD is suppressed from lack of adenylyl cyclase targeting to cilia

doi: 10.1101/2025.05.27.656198

Figure Lengend Snippet: ( A ) Kaplan-Meier survival curves of control (N=77), HoxB7-Cre; Pkd1 f/f (N=41), HoxB7-Cre; Pkd1 f/f ; Ankmy2 f/f (N=37) and HoxB7-Cre; Ankmy2 f/f (N=37) mice. **, p=0024 by log-rank (Mantel-Cox) test. (B) H&E and whole mount images of P15 kidneys in control, HoxB7-Cre; Pkd1 f/f , HoxB7-Cre; Pkd1 f/f ; Ankmy2 f/f , HoxB7-Cre; Ankmy2 f/f mice at P15 in C57BL/6J background. Images of multiple kidneys shown in Figure S3. (C) 2-Kidney to body weight ratios at P15 in HoxB7-Cre; Pkd1 f/f mice were not significantly different from HoxB7-Cre; Pkd1 f/f ; Ankmy2 f/f . Both males (black) and females (red) are shown. (D) At P15 cystic index in HoxB7-Cre; Pkd1 f/f kidneys compared to HoxB7-Cre; Pkd1 f/f ; Ankmy2 f/f were not significantly different. (E) AQP2/pCREB co-staining in kidney sections at P15 shows comparable nuclear pCREB levels in HoxB7-Cre; Pkd1 f/f animals and HoxB7-Cre; Pkd1 f/f ; Ankmy2 f/f cysts. Scale: 50 µm. See also Supplemental Figure 2.

Article Snippet: Sections were incubated with primary antibodies against the following antigens; overnight at room temperature or 4C: Acetylated tubulin (T6793; Sigma mouse IgG2b, 1:500), AQP2 (SC515770, Santa Cruz Biotechnology, mouse IgG1, 1:500), Ki67 (ab16667, Abcam, 1:500), pCREB (9198S; Cell Signaling, 1:500).

Techniques: Control, Staining

Journal: Arthritis Research & Therapy

Article Title: Resistin upregulates fatty acid oxidation in synoviocytes of metabolic syndrome-associated knee osteoarthritis via CAP1/PKA/CREB to promote inflammation and catabolism

doi: 10.1186/s13075-025-03527-6

Figure Lengend Snippet: Resistin promotes the expression of CPT1A through CAP1/PKA/CREB. a In vitro protein and mRNA levels of TLR4 in nMetS-KOA-FLS and MetS-KOA-FLS. b In vitro protein and mRNA levels of CAP1 in nMetS-KOA-FLS and MetS-KOA-FLS. c Immunohistochemistry of CAP1 in synovium of nMetS-KOA and MetS-KOA. d , mRNA and protein levels of CPT1A in MetS-KOA-FLS pretreated with 10 nM TAK-242 for 1 h and then incubated with 200 ng/ml resistin for 24 h. e mRNA and protein levels of CAP1 in MetS-KOA-FLS 48 h after transfection with CAP1-siRNA. f Protein levels of CAP1 and CPT1A in MetS-KOA-FLS transfected with CAP1-siRNA for 48 h and incubated with 200 ng/ml resistin for 24 h. g mRNA levels of CPT1A in MetS-KOA-FLS transfected with CAP1-siRNA for 48 h and incubated with 200 ng/ml resistin for 24 h. h Protein levels of CREB and pCREB ser133 in MetS-KOA-FLS transfected with CAP1-siRNA for 48 h and incubated with 200 ng/ml resistin for 24 h. i mRNA level of CPT1A in MetS-KOA-FLS pretreated with 2.5 µM KG-501 or 2.5 µM H-89 for 1 h and then incubated with 200 ng/ml resistin for 24 h. j Protein levels of CREB, pCREB ser133 , and CPT1A in MetS-KOA-FLS pretreated with 2.5 µM KG-501 or 2.5 µM H-89 for 1 h and then incubated with 200 ng/ml resistin for 24 h. k mRNA levels of CCL3 , ICAM1 and TNFα in MetS-KOA-FLS pretreated with 2.5 µM KG-501 or 2.5 µM H-89 for 1 h and then incubated with 200 ng/ml resistin for 24 h. l mRNA levels of MMP13 and ADAMTS4 in MetS-KOA-FLS pretreated with 2.5 µM KG-501 or 2.5 µM H-89 for 1 h and then incubated with 200 ng/ml resistin for 24 h. Data are expressed as means ± s.e.m. n = 5 biologically independent samples of MetS-KOA-FLS. Statistical significance indicated as * P < 0.05, ** P < 0.01, and *** P < 0.001, as assessed by matched samples t- test

Article Snippet: Antibodies used were as follows: HK2 (Cell Signaling Technology, #2867), LDHA (Cell Signaling Technology, #3582), CPT1A (Cell Signaling Technology, #12252), CAP1 (Abcam, ab155079), TLR4 (WanleiBio, WL00196), CREB (Cell Signaling Technology, #9197), pCREB ser133 (Cell Signaling Technology, #9198), β-actin (Cell Signaling Technology, #4970), Dylight 549, goat anti-rabbit IgG (Abbkine, ABB-A23320).

Techniques: Expressing, In Vitro, Immunohistochemistry, Incubation, Transfection